Table 7.

Effect of BSFLM on amino acid composition in the muscle of fish (g/100 g wet basis) ¹.

Amino acids	Diets							P-value
	SM	BSFLM15	BSFLM30	BSFLM45	BSFLM60	BSFLM75	BSFLM100
EAA
Thr	0.91 ± 0.01	0.88 ± 0.05	0.88 ± 0.06	0.88 ± 0.06	0.90 ± 0.01	0.88 ± 0.04	0.93 ± 0.05	0.69
Val	0.90 ± 0.09	0.91 ± 0.03	0.90 ± 0.02	0.89 ± 0.03	0.89 ± 0.06	0.90 ± 0.07	0.96 ± 0.05	0.69
Met	0.57 ± 0.01	0.55 ± 0.02	0.56 ± 0.04	0.56 ± 0.03	0.56 ± 0.01	0.55 ± 0.02	0.59 ± 0.03	0.35
Ile	0.87 ± 0.03	0.87 ± 0.02	0.87 ± 0.03	0.86 ± 0.04	0.86 ± 0.01	0.87 ± 0.07	0.91 ± 0.03	0.67
Leu	1.61 ± 0.04	1.56 ± 0.06	1.57 ± 0.08	1.56 ± 0.09	1.57 ± 0.01	1.56 ± 0.07	1.65 ± 0.06	0.49
Phe	0.84 ± 0.02	0.81 ± 0.02	0.81 ± 0.04	0.80 ± 0.06	0.83 ± 0.02	0.82 ± 0.04	0.85 ± 0.04	0.58
Lys	1.97 ± 0.02	1.87 ± 0.10	1.92 ± 0.08	1.91 ± 0.09	1.91 ± 0.01	1.89 ± 0.08	1.99 ± 0.07	0.42
His	0.75 ± 0.03	0.73 ± 0.04	0.75 ± 0.03	0.72 ± 0.02	0.73 ± 0.05	0.70 ± 0.04	0.74 ± 0.05	0.61
Arg	1.22 ± 0.04	1.18 ± 0.09	1.21 ± 0.04	1.21 ± 0.06	1.21 ± 0.00	1.16 ± 0.06	1.26 ± 0.06	0.42
NEAA
Asp	2.10 ± 0.02	2.04 ± 0.08	2.08 ± 0.11	2.07 ± 0.12	2.11 ± 0.03	2.04 ± 0.08	2.16 ± 0.12	0.63
Ser	0.83 ± 0.01	0.80 ± 0.05	0.79 ± 0.08	0.79 ± 0.07	0.83 ± 0.02	0.79 ± 0.03	0.83 ± 0.08	0.85
Glu	3.13 ± 0.06	3.01 ± 0.16	3.06 ± 0.15	3.08 ± 0.13	3.07 ± 0.01	2.99 ± 0.13	3.24 ± 0.13	0.30
Gly	0.90 ± 0.02b	0.87 ± 0.05b	0.87 ± 0.02b	0.90 ± 0.04b	0.92 ± 0.03b	0.89 ± 0.04b	1.00 ± 0.07a	0.02
Ala	1.20 ± 0.01	1.14 ± 0.05	1.18 ± 0.05	1.17 ± 0.06	1.19 ± 0.02	1.16 ± 0.05	1.24 ± 0.06	0.23
Cys	0.13 ± 0.09	0.14 ± 0.12	0.19 ± 0.13	0.18 ± 0.12	0.05 ± 0.02	0.06 ± 0.03	0.13 ± 0.11	0.56
Tyr	0.74 ± 0.05	0.71 ± 0.02	0.69 ± 0.09	0.69 ± 0.09	0.75 ± 0.04	0.74 ± 0.04	0.75 ± 0.07	0.66
Pro	0.67 ± 0.03	0.67 ± 0.07	0.68 ± 0.06	0.72 ± 0.03	0.65 ± 0.02	0.64 ± 0.07	0.75 ± 0.04	0.18
∑EAA	9.64 ± 0.21	9.35 ± 0.39	9.49 ± 0.38	9.38 ± 0.43	9.46 ± 0.14	9.32 ± 0.46	9.88 ± 0.35	0.49
∑NEAA	9.69 ± 0.12	9.37 ± 0.49	9.53 ± 0.37	9.61 ± 0.34	9.58 ± 0.13	9.30 ± 0.40	10.09 ± 0.37	0.20
∑EAA:∑NEAA	0.99 ± 0.01	1.00 ± 0.01	1.00 ± 0.01	0.98 ± 0.01	0.99 ± 0.01	1.00 ± 0.01	0.98 ± 0.01	0.14
∑EAA:∑AA	0.50 ± 0.02	0.50 ± 0.03	0.50 ± 0.01	0.49 ± 0.02	0.50 ± 0.01	0.50 ± 0.01	0.49 ± 0.02	0.26
∑AA	19.33 ± 0.29	18.73 ± 0.87	19.00 ± 0.72	18.97 ± 0.76	19.07 ± 0.25	18.63 ± 0.83	19.97 ± 0.70	0.32

BSFLM = black soldier fly larvae meal; SM = soybean meal; Thr = threomine; Val = valine; Met = methionine; Ile = isoleucine; Leu = leucine; Phe = phenylalanine; Lys = lysine; His = histidine; Arg = arginine; Asp = aspartic acid; Ser = serine; Glu = glutamate; Gly = glycine; Ala = alanine; Cys = cystine; Tyr = tyrosine; Pro = proline; EAA = essential amino acids; NEAA = nonessential amino acids; ΣEAA = total essential amino acids; ΣNEAA = total nonessential amino acids; ΣAA = total amino acids.

¹Values are mean ± SD (n = 3). Values with different superscript letters in the same row are significantly different (P < 0.05) from each other.