. 2023 Jul 3;14:425–449. doi: 10.1016/j.aninu.2023.06.006

Table 8.

Effect of BSFLM on fatty acid composition in the muscle of fish (% total fatty acids)¹.

Fatty acids	Diets							P-value
Fatty acids	SM	BSFLM15	BSFLM30	BSFLM45	BSFLM60	BSFLM75	BSFLM100	P-value
C12:0	1.25 ± 0.17^d	1.78 ± 0.29^c	2.13 ± 0.21^bc	2.21 ± 0.08^bc	3.49 ± 0.21^a	3.24 ± 0.36^a	2.53 ± 0.29^b	0.00
C14:0	1.60 ± 0.25^bc	1.54 ± 0.33^c	1.59 ± 0.15^bc	1.91 ± 0.21^abc	2.02 ± 0.25^ab	2.27 ± 0.26^a	2.16 ± 0.21^a	0.01
C16:0	21.11 ± 0.73^bc	20.40 ± 0.44^c	20.20 ± 0.46^c	22.22 ± 1.11^ab	21.30 ± 0.38^abc	22.02 ± 0.30^ab	22.45 ± 0.64^a	0.00
C18:0	6.21 ± 0.74	5.22 ± 0.50	6.03 ± 0.89	5.59 ± 0.19	5.95 ± 0.41	5.81 ± 0.54	5.29 ± 0.43	0.32
∑SFA	28.39 ± 3.85^ab	28.83 ± 1.27^ab	29.52 ± 0.42^b	32.34 ± 2.18^ab	32.70 ± 0.42^a	33.46 ± 0.37^a	32.45 ± 1.25^ab	0.02
C16:1n-7	5.97 ± 0.32^b	7.16 ± 0.56^a	7.18 ± 0.50^a	7.35 ± 0.27^a	6.84 ± 0.30^a	7.54 ± 0.20^a	7.29 ± 0.29^a	0.00
C18:1n-7	2.88 ± 1.06	2.03 ± 0.15	2.64 ± 0.50	2.73 ± 0.86	3.06 ± 1.15	2.75 ± 0.81	2.43 ± 0.75	0.80
C18:1n-9	31.12 ± 2.40	33.65 ± 0.84	31.14 ± 2.84	32.54 ± 0.16	31.64 ± 2.91	31.42 ± 2.84	31.78 ± 2.51	0.82
C20:1n-9	0.72 ± 0.12^d	0.92 ± 0.04^c	1.04 ± 0.04^bc	0.69 ± 0.10^d	1.08 ± 0.04^b	1.09 ± 0.05^b	1.26 ± 0.10^a	0.00
∑MUFA	40.70 ± 1.47	43.75 ± 1.21	42.00 ± 2.85	43.31 ± 1.12	42.62 ± 1.70	42.80 ± 2.31	42.77 ± 2.07	0.59
C18:2n-6	15.33 ± 1.10^ab	16.30 ± 0.59^a	14.25 ± 0.54^b	11.77 ± 1.50^c	11.73 ± 0.57^c	11.37 ± 0.74^c	10.41 ± 1.39^c	0.00
C18:3n-6	0.28 ± 0.07^a	0.19 ± 0.03^ab	0.24 ± 0.04^a	0.21 ± 0.08^a	0.19 ± 0.05^ab	0.11 ± 0.03^bc	0.07 ± 0.03^c	0.00
C20:2n-6	0.86 ± 0.20	0.51 ± 0.42	0.69 ± 0.43	0.70 ± 0.53	0.71 ± 0.12	0.87 ± 0.30	1.19 ± 0.44	0.48
C20:3n-6	2.79 ± 0.36^a	1.51 ± 0.15^c	1.88 ± 0.24^bc	2.00 ± 0.15^bc	2.24 ± 0.36^b	2.39 ± 0.36^ab	2.02 ± 0.26^bc	0.00
C20:4n-6	3.97 ± 0.47	3.47 ± 0.54	4.21 ± 0.43	3.69 ± 0.26	3.32 ± 0.38	3.36 ± 0.50	4.20 ± 0.38	0.09
C22:4n-6	1.72 ± 0.19^b	1.65 ± 0.19^b	2.30 ± 0.25^a	1.93 ± 0.33^ab	2.18 ± 0.07^a	1.98 ± 0.19^ab	2.27 ± 0.19^a	0.01
∑n-6 PUFA	24.94 ± 1.03^a	23.63 ± 1.59^a	23.57 ± 0.64^a	20.30 ± 1.78^b	20.37 ± 0.20^b	20.09 ± 1.82^b	20.17 ± 1.53^b	0.00
C18:3n-3	1.74 ± 0.23^a	1.49 ± 0.06^ab	1.40 ± 0.05^ab	1.45 ± 0.14^ab	1.48 ± 0.04^ab	1.13 ± 0.28^b	1.14 ± 0.28^b	0.01
C20:5n-3	0.44 ± 0.11^c	0.62 ± 0.06^c	0.96 ± 0.13^ab	0.99 ± 0.17^ab	0.91 ± 0.12^b	0.92 ± 0.14^b	1.20 ± 0.25^a	0.00
C22:5n-3	0.25 ± 0.10	0.23 ± 0.06	0.25 ± 0.09	0.19 ± 0.08	0.38 ± 0.13	0.17 ± 0.07	0.27 ± 0.12	0.23
C22:6n-3	1.67 ± 0.06^b	1.62 ± 0.26^ab	1.93 ± 0.24^ab	1.82 ± 0.29^ab	1.87 ± 0.22^ab	1.89 ± 0.26^ab	2.15 ± 0.19^a	0.04
∑n-3 PUFA	4.11 ± 0.14^bc	3.95 ± 0.36^c	4.54 ± 0.33^ab	4.45 ± 0.28^abc	4.64 ± 0.32^ab	4.32 ± 0.38^abc	4.77 ± 0.17^a	0.04
∑PUFA	29.05 ± 3.78	27.59 ± 2.63	28.11 ± 1.87	24.75 ± 4.53	25.01 ± 4.16	24.41 ± 3.48	24.94 ± 3.44	0.53
∑HUFA	10.78 ± 0.34^b	9.10 ± 1.21^c	11.53 ± 0.45^ab	10.62 ± 0.99^b	10.90 ± 0.20^ab	10.72 ± 0.73^b	12.12 ± 0.03^a	0.00
n-3:n-6	0.16 ± 0.01^c	0.17 ± 0.01^c	0.19 ± 0.01^bc	0.22 ± 0.02^ab	0.23 ± 0.02^ab	0.22 ± 0.04^ab	0.24 ± 0.02^a	0.00

BSFLM = black soldier fly larvae meal; SM = soybean meal; ∑SFA = total saturated fatty acids; ∑MUFA = total monounsaturated fatty acid; ∑PUFA = total polyunsaturated fatty acid; ∑HUFA = total highly unsaturated fatty acids; n-3:n-6 = the ratio of ∑n-3 PUFA to ∑n-6 PUFA.

Values are mean ± SD (n = 3). Values with different superscript letters in the same row are significantly different (P < 0.05) from each other.