Table I.
Composition of storage buffers
| Opti-MEM | Krebs-HEPES | WI | Krebs-PSS | |
|---|---|---|---|---|
| K lactobionate, mmol/L | - | - | 100 | - |
| KH2PO4, mmol/L | - | 1.03 | 25 | 2 |
| MgSO4, mmol/L | 0.8 | 1.2 | 5 | 1.2 |
| NaHCO3, mmol/L | 26 | 25 | - | 25 |
| EDTA, mmol/L | - | - | - | 0.026 |
| Raffinose, mmol/L | - | - | 30 | - |
| Adenosine, mmol/L | - | - | 5 | - |
| Glutathione, mmol/L | - | - | 3 | - |
| Allopurinol, mmol/L | - | - | 1 | - |
| Hydroxyl starch, g/L | - | - | 50 | - |
| HEPES, mmol/L | - | 20 | - | - |
| Glucose, mmol/L | 5.5 | 5.6 | - | 11 |
| Na, mmol/L | - | 145 | 25 | 144 |
| K, mmol/L | - | 5 | 120 | 4.7 |
| Cl, mmol/L | - | 108.7 | - | 127.7 |
| Ca, mmol/L | - | 1.8 or 2.5 | - | 2.5 |
| Osmolality, mOsm/L | 280-320 | 320 | ||
| pH | 7.1-7.4 | 7.4 | 7.4 | 7.4 |
| Notes | Proprietary | Insulin, steroid, antibiotics |
EDTA, Ethylenediaminetetraacetic acid; HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; MEM, minimal essential medium; PSS, physiological salt solution.
Arterial storage was tested in physiologic solutions including Opti-MEM at 4 °C and 37 °C, Krebs-HEPES with 1.8 mmol/L calcium and 2.5 mmol/L calcium, and compared Wisconsin buffer. Arterial function was tested in physiologic salt solution (Krebs-PSS).