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Microscopic field (oil immersion objective) showing the accumulation of E. coli at the edge of the urine drop (to the left) with the Gram-stained preparation of 10 μl of a positive urine specimen (≥105 CFU/ml). A DIAPLAN microscope (Leitz, Wetzlar, Germany) and Tri-X pan ISO 400/27 film (Eastman Kodak Company, Rochester, N.Y.) were used.
