Fig. 8.

Effects of co-treatment with HCA and RWP in MASH patients. Macrophages from 8 NASH patients were treated with 500 µM HCA + 200 μg/mL RWP for 24 h. Real-time PCR experiments were performed to evaluate ACLY (A) and ME1 (B) mRNA levels expressed as fold change of unstimulated cells (UNTD). The concentrations of the proinflammatory cytokines IL-6 (C) and IL-1β (D) were determined in free-cell culture supernatants. Data are shown as box plots, where the horizontal line within the boxes is the median, the boxes are the first and third quartiles, and the bars outside the boxes represent the minimum and maximum values. Dots refer to each subject enrolled. Differences were significant according to Student’s t-test (*p < 0.05 and ***p < 0.001)