Table 2.
Epigenome-wide DNA methylation association study identified 8 CpG sites significantly associated with maternal pre-pregnancy body mass index in 903 mother–child pairs from the Boston Birth Cohort
| Chr | CpG | Gene | Relation to Gene | EWAS | ||
|---|---|---|---|---|---|---|
| β (SE)a | P | FDR | ||||
| 6 | cg13694461 | (HLA-Eb) | / | 0.00012 (2.09E − 05) | 4.94E − 08 | 0.036 |
| 15 | cg22940988 | AAGAB | Body | − 0.00032(6.04E − 05) | 1.01E − 07 | 0.037 |
| 4 | cg02266725 | ALPK1 | Body | − 0.00036 (6.80E − 05) | 2.23E − 07 | 0.037 |
| 10 | cg06466203 |
PTEN KILLIN; |
5'UTR TSS200 |
0.00018 (3.50E − 05) | 3.44E − 07 | 0.037 |
| 22 | cg10272744 | LL22NC01-81G9.3 | TSS1500 | − 0.00067 (1.30E − 04) | 3.58E − 07 | 0.037 |
| 8 | cg21701395 | TP53INP1 | 5'UTR | 0.00026 (4.89E − 05) | 2.31E − 07 | 0.037 |
| 6 | cg22307152 | (LINC01625b) | / | − 0.00032 (6.12E − 05) | 3.27E − 07 | 0.037 |
| 5 | cg23151800 | ERCC8 | Body | − 0.00050 (9.78E − 05) | 5.03E − 07 | 0.046 |
Linear regression was fitted with adjustment for child’s sex, maternal age at delivery, maternal race, maternal education level, maternal alcohol consumption, parity, gestational age, estimated cord blood cell composition (CD4 + , CD8 + T cells, B cells, monocytes, granulocytes, natural killer cells, and nucleated red blood cells), and all surrogate variables. CpG = cytosine-phosphoguanine site; Chr = chromosome; EWAS = epigenome-wide association; SE = standard error; FDR = false discovery rate; TSS200 = within 200 bp from transcription start site; TSS1500 = within 1500 bp from transcription start site; Body = the CpG is in gene body; and UTR = untranslated region
aEffect sizes were calculated based on normalized methylation values, denoting the methylation difference per unit increase of maternal BMI
bFor inter-genic CpG sites, we used the UCSC Genome Browser on Human (GRCh37/hg19) to locate the nearest annotated gene