Table 2.
Media used for the pH test
| Treatment | pH | Sucrose (g/L) | Polyethylene glycol 4000 (PEG4000) (g/L) | Boric acid (mg/L) | Calcium nitrate (mg/L) | Germination rate (%) |
|---|---|---|---|---|---|---|
| pH medium 1 | 4.5 | 50 | 100 | 100 | 200 | 24.1 ± 10.9 |
| pH medium 2 | 5.0 | 50 | 100 | 100 | 200 | 30.1 ± 6.3 |
| pH medium 3 | 5.5 | 50 | 100 | 100 | 200 | 52.4 ± 3.7* |
| pH medium 4 | 6.0 | 50 | 100 | 100 | 200 | 66.9 ± 4.7* |
| pH medium 5 | 6.5 | 50 | 100 | 100 | 200 | 53.6 ± 5.2* |
Germination rate is the mean ± SD of five replicates. * Indicates the first echelon of multiple comparison results. ANOVA showed that the germination rate was significantly higher at pH 5.5, 6.0 and 6.5 than at 4.5 and 5.0 (P < 0.001)