Table 6.
Media used for the Calcium nitrate concentration test
| Treatment | pH | Sucrose (g/L) | Polyethylene glycol 4000 (PEG4000) (g/L) | Boric acid (mg/L) | Calcium nitrate (mg/L) | Germination rate (%) |
|---|---|---|---|---|---|---|
| Calcium medium 1 | 6.0 | 50 | 50 | 100 | 0 | 7.9 ± 2.4 |
| Calcium medium 2 | 6.0 | 50 | 50 | 100 | 100 | 61.7 ± 5.7* |
| Calcium medium 3 | 6.0 | 50 | 50 | 100 | 200 | 71.9 ± 4.1* |
| Calcium medium 4 | 6.0 | 50 | 50 | 100 | 300 | 79.7 ± 4.6* |
| Calcium medium 5 | 6.0 | 50 | 50 | 100 | 400 | 61.6 ± 9.4* |
Germination rate is the mean ± SD of five replicates. * Indicates the first echelon of multiple comparison results. Based on ANOVA, the mean germination rates differed significantly between the media supplemented with and without Calcium nitrate (P < 0.01), while the differences between the four different Calcium nitrate concentrations were not significant