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. 2023 Aug 23;25:154. doi: 10.1186/s13075-023-03110-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Fibroblast (CD45−PDPN+) subsets in advanced OA synovium. A, B Relative frequency (%) of fibroblast subsets based on fibroblast activation protein (FAP) and CD90 expression, n=10; C table with relative frequency (%) of fibroblast subsets based on expression of FAP/CD90 and CD34/CD90. D, E Relative frequency of fibroblast subsets based on CD34 and CD90 expression, n=8; F–I Relationship between fibroblast subsets as the percentage of all viable cells and mean compartmental Kellgren-Lawrence (KL) grade or immune (CD45+) cells as the percentage of all viable cells. J Immunofluorescence staining of fibroblast (activation) markers podoplanin (PDPN, green), FAP (red), CD90 (pink), and CD34 (yellow) in advanced OA synovium; DAPI staining (blue) was used to visualise nuclei