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. 2023 Aug 23;23:31. doi: 10.1186/s12896-023-00804-7

Fig. 3.

Fig. 3

Analytical sensitivity of the method. (A) Serial dilutions (1:10) of mixes containing 1–10.000 genome copies of different pathogens (0.04–100 pg) were tested with the assay. 450 ng (104 copies) of human genomic DNA were spiked to all mixes. Each colored symbol represents one pathogen or AMR included in the panel. Error bars correspond to standard deviation (n = 4). Dashed line indicates the set sensitivity limit of 10 UMIs/pathogen. (B) Examples of UMI counts for individual probes for the selected pathogens Candida albicans, Pseudomonas aeruginosa and Staphylococcus aureus (n = 3)