Table 3.
sEH inhibitory activity, type of inhibitions, and inhibition constants of all isolated compounds (1–24).
| Compound | Inhibition rate at 100 µM (%) | IC50 (μM) | Inhibition type | Ki (μM) |
|---|---|---|---|---|
| 1 | 34.76 ± 0.2 | − | − | − |
| 2 | 35.26 ± 2.4 | − | − | − |
| 3 | 5.91 ± 1.6 | − | − | − |
| 4 | 0.74 ± 0.9 | − | − | − |
| 5 | 40.36 ± 0.7 | − | − | − |
| 6 | 60.16 ± 6.3 | 71.73 ± 0.49 | − | − |
| 7 | 50.02 ± 1.9 | 93.75 ± 1.94 | − | − |
| 8 | 23.13 ± 1.5 | − | − | − |
| 9 | 40.71 ± 0.5 | − | − | − |
| 10 | 19.05 ± 1.4 | − | − | − |
| 11 | 42.52 ± 0.6 | − | − | − |
| 12 | 23.85 ± 3.3 | − | − | − |
| 13 | 11.46 ± 0.2 | − | − | − |
| 14 | 53.67 ± 0.5 | 94.03 ± 2.04 | − | − |
| 15 | 53.15 ± 0.1 | 87.34 ± 1.88 | − | − |
| 16 | 53.03 ± 3.1 | 97.81 ± 1.76 | − | − |
| 17 | 62.66 ± 5.8 | 67.74 ± 3.07 | − | − |
| 18 | 12.88 ± 0.2 | − | − | − |
| 19 | 93.47 ± 0.9 | 18.58 ± 2.42 | Competitive | 20.9 |
| 20 | 90.88 ± 0.1 | 25.95 ± 2.01 | Competitive | 11.1 |
| 21 | 67.59 ± 2.7 | 46.35 ± 2.72 | Competitive | 23.3 |
| 22 | 85.59 ± 0.8 | 23.56 ± 2.94 | Competitive | 12.8 |
| 23 | 90.32 ± 0.9 | 14.24 ± 0.91 | Competitive | 9.7 |
| 24 | 76.65 ± 1.1 | 39.58 ± 3.74 | Non-competitive | 23.1 |
| AUDAa | 33.68 ± 1.95 nM |
All data are expressed as the mean ± SEM of three independent experiments.
aPositive control.
(−) No test.