Table 8.

List of toxicological studies conducted with 3’‐SL produced by enzymatic synthesis

Test material	Reference	Type of study	Experimental details
3’‐SL (98.8%)	Kim et al. (2018)	Bacterial Reverse Mutation Test	5, 10, 50, 100, 250, 500, 1,000, 2,500 and 5,000 μg 3’‐SL mL; ± S9
		In vitro chromosome aberration test (CHL)	5, 10, 50, 100, 250, 500, 1,000, 2,500 and 5,000 μg 3’‐SL/mL; ± S9
		In vivo mammalian Cell Micronucleus Test	500, 1,000 and 2,000 mg 3’‐SL/kg bw
		Acute Toxicity Study in the SD Rat	5 SD rats/sex per group; 5,000, 10,000, 15,000 and 20,000 mg 3’‐SL/kg bw per day
		28‐day GLP repeated dose oral toxicity study in the SD rat	10 SD rats/sex per group; 500, 1,000, 2,000 mg 3’‐SL/kg bw per day
		90‐day GLP repeated dose oral toxicity study in the SD rat	10 SD rats/sex per group; 500, 1,000, 2,000 mg 3’‐SL/kg bw per day

CHL: Chinese hamster lung; SD: Sprague‐Dawley.