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. 2023 Aug 10;19(8):e1011571. doi: 10.1371/journal.ppat.1011571

Fig 6. Removal of the MUC1 extracellular domain increases spike and virus attachment.

Fig 6

(A) Immunofluorescence confocal microscopy of Calu-3 cells incubated with 2.5 ug/ml SARS-CoV-2 spike (Fc-tagged SARS2-S1B-Fc, red) at 4°C for 1 h. Spike was incubated with the cell without permeabilization. Increased spike binding and higher spike signal intensity was observed after treatment with StcE in comparison to E447D treatment and control. White scale bars represent 20 μm (B) Quantification of spike fluorescence signal as depicted in A. Fluorescence intensity along the edge of cell island was determined in control, StcE- and E447D-treated cells using ImageJ. Mean ± SEM raw integrated density/length from three random fields from three independent experiments are plotted. The area of spike binding was significantly higher in StcE-treated cells. White scale bars represent 50 μm.