Fig 2. FCCP-induced mitochondrial fragmentation in WT and yta4Δ cells.

(A) Maximum projection time-lapse images of WT and yta4Δ cells expressing Sdh2-mCherry (also see S4–S7 Movies). Cells treated with FCCP at the indicated concentrations (0 μM and 0.5 μM) in profusion chambers were imaged immediately after the treatment. Scale bars: 10 μm. (B) Quantification of the mitochondrial number of WT and yta4Δ cells that were or were not treated with FCCP. Time-lapse movies as indicated in (A) were used for the quantification, and 20 cells at each time point were analyzed. Data represent mean ± SD. For the comparison of the effect of 2 treatments (i.e., 0 or 0.5 μM) on WT or yta4Δ cells, the Wilcoxon–Mann–Whitney rank sum test was performed to calculate the p values of the 2 sets of values at each time point. To test the effect of 0.5 μM FCCP treatment on WT or yta4Δ cells over time, Friedman test was performed to calculate the p values (indicated as F: p in the graphs). In addition, in the right graph (yta4Δ cells treated with 0.5 μM FCCP), the Wilcoxon–Mann–Whitney rank sum test was performed to calculate the p values of the comparisons between 0-min and other time points. Experiments were repeated twice, and raw data are available in S1 Data. (C) Maximum projection time-lapse images of Sdh2-mCherry-expressing WT and yta4Δ cells treated with 2 μM FCCP. Note that mitochondria fragmented rapidly both in WT and yta4Δ cells after the cells were treated with 2 μM FCCP. Scale bars: 10 μm. WT, wild-type.