Figure 5. A high level of CD8⁺Trm in erosive oral lichen planus (EOLP) is closely related to the basement membrane and directly affects oral lichen planus’s (OLP) clinical manifestations through active secretion.

(A, B) Immunofluorescence images of CD8⁺ Trm cells in non-erosive oral lichen planus (NEOLP; the white dotted line is the basement membrane, and the red arrow points to the CD8⁺ TRM cells). (C, D) Immunofluorescence images of CD8⁺ Trm cells in EOLP (the white dotted line is the basement membrane, and the red arrow points to the CD8⁺ Trm cells). (E) Schematic diagram of CD8⁺ Trm sorting by flow cytometry. (F) Image of sorting CD8⁺ Trm flow cytometry and gating strategy. (G) Comparison of the proportion of CD8⁺ Trm in NEOLP versus EOLP. (H) Enzyme-linked immunosorbent assay (ELISA) detection results of CD8⁺ TRM secreting TNF-α, IFN-γ, and IL17 in NEOLP versus EOLP.