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. 2023 Aug 14;2(8):pgad261. doi: 10.1093/pnasnexus/pgad261

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© The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Y1R and MOR are coexpressed in the DRG and DH. A–D) FISH demonstrating colocalization of Oprm1 and Npy1r in the same cells in both lumbar DRG A and B) and DH C and D). B and D) represent zoomed in box insets for A and C, respectively. Arrows indicate colocalization. E) Npy1r colocalizes with Oprm1 in the DRG (Npy1r/Oprm1—54.08 ± 2.85%; Oprm1/Npy1r—75.54 ± 2.87%). F) Npy1r colocalizes with Oprm1 in the spinal cord DH (Npy1r/Oprm1—51.96 ± 1.59%; Oprm1/Npy1r—32.28 ± 1.22%). Averages are determined from 4 to 5 quantified sections/mouse, n = 3 mice/group. Data are shown as mean ± SEM.