Table 3.

Primer sequences used for the generation of various genetic deletions in PAO1 strains

Name	Sequence	Targeted gene
607_JB_PA_m_toxA_R1 Fw	5′-CCC​AGT​CTC​GAG​GTC​GAC​GGT​ATC​GAT​AAG​CTT​GAT​ATC​GAA​TTC​GGC​CGA​CGG​CGG​C-3′	toxA
608_JB_PA_m_toxA_R1 Rv	5′-TCG​CGA​TGC​ACC​TGA​CAC​CCG​AGG​ACC​TGA​AGT​AAC​TGC​CGC-3′	toxA
609_JB_PA_m_toxA_R2 Fw	5′-GGC​AGT​TAC​TTC​AGG​TCC​TCG​GGT​GTC​AGG​TGC​ATC​GC-3′	toxA
610_JB_PA_m_toxA_R2 Rv	5′-CTG​GAG​CTC​CAC​CGC​GGT​GGC​GGC​CGC​TCT​AGA​ACT​AGT​GGA​TCC​AGC​CAT​TGT​TCG​ACG​AAT​AAA​GCC​ACC-3′	toxA
611_JB_PA_m_toxA_Check R1	5′-GAA​GTA​CTT​CAA​CGG​GTT​GAT​CCC​C-3′	toxA
612_JB_PA_m_toxA_Check R2	5′-CGT​TTC​CGC​AAC​GCT​TGA​AGT-3′	toxA
EM019-xcpQ R1 Fw	5′-TTC​CAC​ACA​TTA​TAC​GAG​CCG​GAA​GCA​TAA​ATG​TAA​AGC​AAG​CTT​ACG​ATA​AAG​ACC​AGG​AGT​GAT​GTA​TTG​CC-3′	XcpQ
EM020-xcpQ R1 Rv	5′-CGC​CGT​TAT​TCC​GTC​ATC​AGC​AAA​GGC​TGG​GAC​ATC​GG-3′	XcpQ
EM021-xcpQ R3 Fw	5′-ACC​CGA​TGT​CCC​AGC​CTT​TGC​TGA​TGA​CGG​AAT​AAC​GGC​GCC-3′	XcpQ
EM022-xcpQ R3 Rv	5′-GGA​AAT​TAA​TTA​AGG​TAC​CGA​ATT​CGA​GCT​CGA​GCC​CGG​GGA​TCC​ACG​CCT​GGT​TCG​TGG​C-3′	XcpQ
EM023-xcpQ R1 check	5′-CCC​GGC​CAG​TCA​CAC​CTA​TTG​AT-3′	XcpQ
EM024-xcpQ R3 check	5′-AGT​GCT​TGC​CGA​CAA​CGA​CC-3′	XcpQ