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Immunohistochemistry of ELN, COL II and ACAN in presence and absence of oxygen tension. (A) Negative control was illustrated where primary antibody was replaced by PBS (a∼c). (B∼D) Type 2 collagen, aggrecan and elastin expression was detected in auricular perichondrocytes and bone marrow-derived mesenchymal stem cells in presence of high (a∼c, g∼i) and low oxygen tension (d∼f, j∼l), respectively (scale bar 200 μm).
