Fig. 5.

Quantification of Neu5Gc in the hiPSC-CM lysate using LC/MRM MS. The total sialic acid content (Ne-u5Ac and Neu5Gc) was significantly higher at 2 to 6 weeks (W) after differentiation into hiPSC-CM than at 0 weeks (hiPSCs) under both (A) XC condition and (B) XF conditions. Neu5Gc was only detected in XC-hiPSC-CM, even though the most prominent increase in the level of sialic acid was attributed to Neu5Ac. Statistical analysis was performed using the Student’s t-test. *p<0.05, ***p<0.001. Values are presented in terms of means±SEM. n=3 per group. N.D., not detected.