Figure 4.

2-photon rig and anesthetic support set up. (A) Schematic depiction of key elements of the microscope body, highlighting the large range of motion for accommodating a large animal. (B) Schematic of room layout during an imaging session. (C) Photograph of an imaging experiment with an 84 kg pig under the 2-photon microscope. The surgery table was resting on and mechanically coupled to the optical table. The ventilator is placed perpendicular to the optical table to the left of the pig with the ECG, gas analyzers, physiology monitor (not shown) and IV pumps on the ventilator (scale: human = 1.7 m). (D) Photograph of overall microscope construction.