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. 2023 Aug 29;13:14158. doi: 10.1038/s41598-023-40638-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Imaging in vivo intraneuronal chloride of the cortex. (A) The heartbeat triggered acquisition of a fast z-stack spanning 600 × 600 × 400 µm provided stable images of the fluorescent protein-transduced neurons in vivo. Because these volumes were acquired between heartbeats, individual iterations (first 3 columns) could be averaged together to increase dynamic range and decrease shot noise in the final image stacks (4th column). (B) Merging the cyan and yellow fluorescent protein signals from SuperClomeleon provides a pseudo-colored imaged of intracellular chloride.