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. 2023 Aug 29;6:889. doi: 10.1038/s42003-023-05226-x

Fig. 3. PANX1 knockout alleviates mice kidney I/R injury.

Fig. 3

a Serum creatinine (Scr) levels of PANX1−/− mice and WT mice subjected to sham or I/R treatment (n = 6). b NGAL mRNA levels of PANX1−/− mice and WT mice subjected to sham or I/R treatment (n = 3). c KIM-1 mRNA levels of PANX1−/− mice and WT mice subjected to sham or I/R treatment (n = 3). d Representative histological H&E staining and TUNEL staining show necrotic areas in kidneys from PANX1−/− mice and corresponding WT mice subjected to sham or I/R operation (Bar = 50 μm). e Tubular injury scores and TUNEL-positive cells rate (n = 6). f, g The protein levels of apoptosis-related protein in PANX1−/− and WT mice subjected to sham or I/R challenge (n = 5). h, i Representative histogram showing apoptosis for HK2 and PANX1KD cells from the indicated groups measured by flow cytometry (n = 5). j, k The protein levels of apoptosis-related protein in PANX1KD and PANX1OE cells subjected to normal or H/R challenge (n = 5). GAPDH served as the loading control. Results are presented as means ± SEM. P values from Student’s t test. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001.