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. 2023 Aug 29;6:889. doi: 10.1038/s42003-023-05226-x

Fig. 4. PANX1 deficiency alleviates oxidative stress and mitochondrial damage in hypoxia/reoxygenation HK2 cells.

Fig. 4

a Immunofluorescence of ROS from PANX1KD and HK2 cells after H/R injury (Bar = 20 μm). b, c Representative histogram showing mitochondrial ROS for HK2 and PANX1KD cells from the indicated groups measured by flow cytometry (n = 5). d, e Representative mitochondrial membrane potential (MMP) in H/R or normal HK2 cells from the indicated groups measured by flow cytometry (n = 5). f Representative images of transmission electron microscopy in different groups, arrows represent mitochondria undergoing mitophagy (Bar = 5 μm). g, h DHE staining for ROS in different groups (Bar = 20 μm) (n = 5). Results are presented as means ± SEM. P values from Student’s t test. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001.