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. 2023 Aug 29;9:90. doi: 10.1038/s41421-023-00583-7

Fig. 5. NRBF2 deficiency impairs the autophagosome formation and neurogenesis of aNSCs.

Fig. 5

a–d Immunoprecipitation (IP) and western blotting analysis (a) of the interaction between NRBF2 (b), VPS34 (c), and Beclin1 (d) with ATG14L in control and NRBF2-deficient aNSCs (n = 7 independent experiments per group). e Quantification of ATG14L-immunoprecipitated VPS34 kinase activity (n = 8 independent experiments per group). f–h Representative images of western blotting analysis (f) and quantification of LC3-II (g) and p62 (h) in control and NRBF2-deficient aNSCs treated with vehicle (0.1% DMSO) or bafilomycin A1 (BafA1) (n = 9 independent experiments per group). i–k Representative images (i) and quantitation of GFP-RFP-LC3 puncta (j) or RFP-LC3 puncta (k) (n = 21–25 cells per group). Higher magnification views of selected regions (white rectangle). Scale bars, 5 μm (overview) and 2 μm (zoom). Data are presented as means ± SEM and analyzed by two-sided unpaired t-test (b–e) or two-way ANOVA followed by Bonferroni’s post hoc test (g, h, j, k). *P < 0.05, **P < 0.01, and ***P < 0.001. NS indicates no significant difference.