Table 3.
Motor parameters estimated by MATLAB “bayesopt” parameter optimization function using the limits shown for each parameter
| Parameter | Limits | Optimized parameter Values |
Final value | |
|---|---|---|---|---|
| Seven-parameter fit | Three-parameter fit | |||
| Kinesin-1 detachment force | 0.01–50 pN | 17–49 pN | ideal bond | ideal bond |
| DDB detachment force | 0.01–50 pN | 5–49 pN | ideal bond | ideal bond |
| Kinesin-1 reattachment rate | 0.01–100 s−1 | 30–96 s−1 | 50 s−1 | 50 s−1 |
| DDB reattachment rate | 0.01–100 s−1 | 0.3–86 s−1 | 5 s−1 | 5 s−1 |
| DDB stall force | 0.01–6 pN | 5.7–6 pN | 5.9–6 pN | 6 pN |
| DDB backstepping rate | 1–20 s−1 | 1–6 s−1 | 1–4 s−1 | 1 s−1 |
| Motor stiffness | 0.01–0.3 pN/nm | 0.2–0.3 pN/nm | 0.18–0.22 pN/nm | 0.2 pN/nm |
Parameter ranges from 10 independent optimization runs are shown. From the seven-parameter fit, four parameters were fixed at the values shown, and the three remaining parameters were then optimized. Final values refer to parameter values chosen for the best-fit model.