Figure 2.

Fibrinogen coverage has no effect on force generation. (A) Schematic representation of the preparation of micro-patterned gel surfaces with varying fibrinogen coverage (100%, 25%, 10%) by photo patterning (LIMAP). (B) Average total force curves for blood platelets (n$\ge$ 15) upon stimulation by thrombin (0.1 U/mL) on gels covered by various concentrations of fibrinogen (dotted lines); the SEs are included as the transparent areas. Averaged exponential fits are shown as solid lines. Each dataset comprising the averaged curves is fitted and the maximum forces and time constants are determined. (C) Maximum forces for each dataset determined from the averaged total force curves shown in (B). (D) Time constants for each dataset determined the averaged total force curves shown in (B). The violin plot outlines illustrate the kernel probability density; the width of the shaded area represents the proportion of the data points located there. In each violin plot, the white circle indicates the median, the thick gray bar indicates interquartile range, and the thin gray line indicates the full range of distribution without outliers. To see this figure in color, go online.