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. 2023 May 15;13(8):3365–3381. doi: 10.1016/j.apsb.2023.05.010

Figure 3.

Figure 3

Expression of PIEZO2 in DRG neurons of VCR-induced neuropathic pain rats. (A) Relative mRNA expression of Piezo2 in DRG of control rats (Cont) and VCR-induced pain model rats (VCR). Representative (B) and summary (C) data of PIEZO2 and Tubulin expression with Western blot assay in DRG of control rats (Cont) and VCR-pain model rats (VCR), respectively. β-Actin was used as control. n = 12 each group. Representative images (D) and IF intensity (E) of PIEZO2-positive DRG neurons of control rats (Cont) and VCR-pain model rats (VCR). n = 254 and 174 in Cont and VCR group, respectively. (F) The ratio of PIEZO2-positive DRG neurons to total DRG neurons in control rats (Cont) and VCR-pain model rats (VCR). The amounts of PIEZO2-positive DRG neurons and total DRG neurons of control rats (Cont) and VCR-pain model rats (VCR) are shown above the bars. Data are shown in mean ± SEM; ∗P < 0.05, ∗∗∗P < 0.001.