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. 2023 May 15;13(8):3365–3381. doi: 10.1016/j.apsb.2023.05.010

Figure 5.

Figure 5

VCR treatment affected the properties of PIEZO2 MA currents. (A) Representative traces of PIEZO2 MA currents elicited by minimum membrane displacements at the initial time and 30 min after establishing whole-cell mode patch recordings with (VCR) or without (Cont) intracellular administration of VCR (5 μmol/L). (B) Mechanical thresholds for evoking PIEZO2 MA currents at initial time (solid bars) and 30 min (open bars) with (VCR) or without (Cont) intracellular administration of VCR. n = 16 (Cont initial), 16 (Cont 30 min), 9 (VCR initial) and 8 (VCR 30 min), respectively. Representative traces of PIEZO2 MA currents elicited by a 7-μm membrane displacement at the initial time (top) and 30 min (bottom) without (left, Cont) (C) or with (right, VCR) (D) VCR treatment. The inactivation kinetics were fitted with single exponential function (red line) and the decay time constants (τ) were shown. The interval between dash lines (bold arrow) show the onset latency for evoking PIEZO2 MA currents. (E) Summary of the latency for evoking PIEZO2 MA currents at initial time (solid bars) and 30 min (open bars) with (VCR) or without (Cont) VCR treatment. n = 11 (Cont initial), 20 (Cont 30 min), 15 (VCR initial) and 8 (VCR 30 min), respectively. (F) Summary of decay time constants of PIEZO2 MA currents at initial time (solid bars), 16 min (gray bars) and 30 min (open bars) with (VCR) or without (Cont) VCR treatment. n = 39 (Cont initial), 38 (Cont 16 min), 31 (Cont 30 min), 11 (VCR initial), 12 (VCR 16 min) and 11 (VCR 30 min), respectively. Data are shown in mean ± SEM; ∗P < 0.05, ∗∗∗P < 0.001, ns indicates no significant difference.