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. 2023 May 15;13(8):3365–3381. doi: 10.1016/j.apsb.2023.05.010

Figure 6.

Figure 6

VCR mimicked the effects of osmotic swelling on PIEZO2 MA currents in DRG neurons. Images (top) and time courses (bottom) of cell swelling induced by hypertonic internal solution (420 mOsm, n = 10) (A) and pipette application of VCR (5 μmol/L, n = 10) (B) after establishing the whole-cell mode patch recording over time. The position of the recording electrode and mechanical stimulation probe are indicated by a white and a black star, respectively. (C) Sample traces show PIEZO2 MA currents at 30 min after establishing whole-cell mode patch recording with hypertonic internal solution (420 mOsm). At that time, cells were perfused with isotonic bath solution (black line) or hypertonic bath solution (420 mOsm, gray line). Membrane displacement was 7 μm (above). (D) Summary data of the potentiation of PIEZO2 MA currents over time using hypertonic internal solution (Hypertonic). In the last 6 min, cells were perfused with isotonic bath solution (n = 14) and hypertonic bath solution (420 mOsm, gray stripe, n = 5), respectively. (E) Similar to (C) but the sample traces of PIEZO2 MA currents were recorded using isotonic internal solution containing 5 μmol/L VCR (Isotonic + VCR). (F) Summary data of the potentiation of PIEZO2 MA currents over time using isotonic internal solution contained 5 μmol/L VCR (Isotonic + VCR). In the last 6 min, cells were perfused with isotonic bath solution (n = 10) and hypertonic bath solution (420 mOsm, gray stripe, n = 4), respectively. Data are shown in mean ± SEM; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗/###P < 0.001.