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. 2023 May 15;13(8):3365–3381. doi: 10.1016/j.apsb.2023.05.010

Figure 9.

Figure 9

Disruption but not stabilization of microtubules potentiated PIEZO2 MA currents in DRG neurons. Sample traces (top) and time courses (bottom) of PIEZO2 MA currents evoked by a 7-μm membrane displacement after establishing whole-cell mode patch recording with isotonic internal solution containing 5 μmol/L NDZ (A) or 1 μmol/L TAXEL (B) over time. In the top panels in (A) and (B), the traces of PIEZO2 MA currents at the initial time and 30 min are indicated with black line and gray line, respectively. n = 14 (Isotonic) and 8 (Isotonic + NDZ) in (A); n = 13 (Isotonic) and 9 (Isotonic + TAXEL) in (B), respectively. The mechanical thresholds (C) and the onset latency (D) for evoking PIEZO2 MA currents at initial time (solid bars) and 30 min (open bars) after establishing whole-cell mode patch recording with isotonic internal solution (Cont), or isotonic internal solution containing NDZ (NDZ) or isotonic internal solution containing TAXEL (TAXEL) were measured. n = 9 (Cont initial), 7 (Cont 30 min); n = 9 and 9 (NDZ initial), 6 and 8 (NDZ 30 min), 11 and 9 (TAXEL initial) and 6 and 8 (TAXEL 30 min) in (C) and (D), respectively. (E) The SPMT was measured over time among the above 3 groups of DRG neurons (square, Cont, n = 11; circle, NDZ, n = 8; and triangle, TAXEL, n = 8). BL, baseline before establishing the whole-cell mode patch. Data are shown in mean ± SEM; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.