Figure 4.

A single LV-DEN injection induces robust polyfunctional CD8⁺ T-cell responses. IFNAR-BL6 mice were injected i.m. with LV_IND-DEN or LV_IND-GFP (n = 2/group) at day 0. At day 14, splenocytes were collected, pooled by group, and analyzed by ICS. (A) Gating strategy to identify CD8⁺ and CD4⁺ T cells. (B) Identification of CD8⁺ (left panel) or CD4⁺ T cells (right panel) expressing IFNγ (upper panels), co-expressing IFNγ and TNF/IL-2 (middle panels), or co-expressing IFNγ and the degranulation marker CD107a (lower panels) among each spleen T subset. Splenocytes were stimulated either with an irrelevant control peptide (left) or with a pool of 11 DEN-specific peptides ( Table S1 ) derived from immunogenic regions of NS3-3, NS4B-1, NS4A, NS5-2, and NS5-4 (center and right).