. 2023 Aug 29;17:92–100. doi: 10.33393/dti.2023.2613

TABLE II -.

Primer sequences and characteristics of selected genes

Gene names	Nucleotide sequences (5ʹ → 3ʹ)	Hybridization temperature	Amplicon size	Reference
Adhesin genes				22
bfp	GAC ACC TCA TTG CTG AAG TCG	57°C	324 bp
bfp	CCA GAA CAC CTC CGT TAT GC	57°C	324 bp
eae	TCA ATG CAG TTC CGT TAT CAG TT	65°C	494 bp
eae	GTA AAG TCC GTT ACC CCA ACC TG	65°C	494 bp
ipaH	GAA AAC CTC CTG GTC CAT CAG G	53°C	424 bp
ipaH	GCC GGT CAG CCA CCC TCT GAG AGT AC	53°C	424 bp
Eagg	ACG CAG AGT TGC CTG ATA AAG	53°C	630 bp
Eagg	AAT ACA GAA TCG TCA GCA TCA GC	53°C	630 bp
Toxin genes
slt1	TTT ACG ATA GCA TTC TCG AC	56°C	130 bp
slt1	CAC ATA TAA ATT ATT TCG CTC	56°C	130 bp
slt2	CTT CAC GTC ACC ATA CAT AT	56°C	346 bp
slt2	ACG ATG TGG TTT ATT CTG GA	56°C	346 bp
lt	GGC GAC AGA TTA TAC CGT GC	56°C	707 bp
lt	CCG AAT TCT GTT ATA TAT GTC	56°C	707 bp
sta	TTA ATA GCA CCC GGT ACA AGC AGG	43°C	146 bp
sta	CTT GAC TCT TCA AAA GAG AAA ATT AC	43°C	146 bp
Antibiotic resistance genes
int1	ACATGTGATGGCGACGCA CGA	57°C	580 bp
int1	ATTTCTGTCCTGGCTGGC GA	57°C	580 bp
int2	GTAGCAAACGACTGACGAAAT G	62°C	806 bp
int2	CACGGATATGCGACAAAA AGG T	62°C	806 bp
int3	GCC CCG GCA GCG ACT TTC AG	62°C	1200 bp
int3	ACG GCT CTG CCA AAC CTG ACT	62°C	1200 bp
SHV	TTATCTCCCTGTTAGCCACC	55°C	800 bp
SHV	GATTTGCTGATTTCGCTCGG	55°C	800 bp
Tem	ATAAAATTCTTGAAGACGAAA	55°C	850 bp
Tem	GACAGTTACCAATGCTTAATC	55°C	850 bp
catA1	CCTGCCACTCATCGCAGTAC	57°C	450 bp
catA1	CTGCCTGGACAACATTGCTT	57°C	450 bp
QnrA	TCAGCACAAGAGGATTTCTC	55°C	657 bp
QnrA	GGCAGCACTATTACTCCCA	55°C	657 bp
QnrB	GATCGTGAAAGCCAGAAAGG	55°C	469 bp
QnrB	ACGATGCCTGGTAGTTGTCC	55°C	469 bp
QnrS	ACGACATTCGTCAACTGCAA	55°C	417 bp
QnrS	TAAATTGGCACCCTGTAGGC	55°C	417 bp
Carbapenemase				27
bla_KPC	CATTCAAGGGCTTTCTTGCTGC	55°C	538 bp
bla_KPC	ACGACGGCATAGTCATTTGC	55°C	538 bp
bla_OXA-48	GCTTGATCGCCCTCGATT	55°C	281 bp
bla_OXA-48	GATTTGCTCCGTGGCCGAAA	55°C	281 bp
bla_NDM-1	ATGGAATTGCCCAATATTATGCAC	55°C	813 bp
bla_NDM-1	TCAGCGCAGCTTGTCGGC	55°C	813 bp