Reactivities of pooled and individual serum samples from P. marneffei-infected patients to P. marneffei CYA by Western blotting. Lanes 1 and 2, reactivities of sera in pools Pm1 and Pm2, respectively (pooled sera were used at 1:100, and peroxidase-conjugated goat anti-human IgG polyclonal sera were used at 1:1,000); lanes 3 to 23, reactivities of individual sera from P. marneffei-infected patients (sera were used at 1:100, and peroxidase-conjugated goat anti-human IgG polyclonal sera were used at 1:1,000) to P. marneffei CYA; lane 24, negative control (no primary antibody). Molecular masses (on the left) are in kilodaltons.