FIG. 3.

Immunoenzyme development of Western blots of fraction 11 obtained by IEF with various pooled sera. Lanes A and B, reactivities of pooled normal human sera (n = 30); lanes D and E, pooled sera from P. marneffei-infected patients (pool Pm2; n = 21); lanes G and H, pooled sera from histoplasmosis patients (n = 11); lanes K and L, pooled sera from candidiasis patients (n = 9); lanes N and O, pooled sera from aspergillosis patients (n = 20); lanes Q and R, pooled sera from cryptococcosis patients (n = 10); lanes C, F, I, M, P, and S, molecular mass markers; lanes J and T, duplicates of Coomassie blue-stained fraction 11 obtained by IEF. Pooled sera were used at a dilution of 1:1,000, and the peroxidase-conjugated goat anti-human IgG peroxidase conjugate was used at 1:1,000. Molecular masses (on the left) are in kilodaltons.