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Purification of the 61-kDa (A) and 54-kDa (B) P. marneffei antigens as monitored by silver staining. Lane A, CYA; lane B, fractions 10 and 11 (pooled) obtained with the Rotofor system; lane C, purified antigen after preparative SDS-PAGE (61- or 54-kDa antigen as annotated on the right). Molecular masses (on the left) are in kilodaltons.
