KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-Helios | Cell Signaling Technology | Cat#42427; RRID:AB_2799221 |
| Goat polyclonal anti-Helios | Santa Cruz Biotechnology | Cat#sc-9864; RRID: AB_2124965 |
| Rabbit monoclonal anti-Ikaros | Cell Signaling Technology | Cat#9034; RRID: AB_2797691 |
| Rabbit monoclonal anti-Aiolos | Cell Signaling Technology | Cat#15103; RRID: AB_2744524 |
| Rabbit polyclonal anti-IKZF4 | Abcam | Cat#ab220329; RRID: NA |
| Rabbit polyclonal anti-CMYC | Cell Signaling Technology | Cat# 5605S; RRID: 1903938 |
| Rabbit polyclonal anti-HOXA9 | Abcam | Cat# ab140631; RRID:NA |
| Rabbit monoclonal anti-p21 Waf1/Cip1 | Cell Signaling Technology | Cat#2947; RRID:823586 |
| Rabbit monoclonal anti-p53 | Cell Signaling Technology | Cat#2527; RRID: AB_10695803 |
| Rabbit monoclonal anti-MDM2 | Cell Signaling Technology | Cat#86934; RRID: AB_2784534 |
| Rabbit monoclonal anti-casein kinase 1 alpha | Abcam | Cat#ab206652; RRID: AB_2925161 |
| Rabbit polyclonal anti-eRF3a/GSPT1 | Proteintech | Cat#10763-1-AP; RRID: AB_2115506 |
| Rabbit polyclonal anti-CRBN | Sigma-Aldrich | Cat#HPA045910; RRID: AB_10960409 |
| Mouse monoclonal anti-β actin | Santa Cruz Biotechnology | Cat#sc-47778; RRID: AB_2714189 |
| Goat anti-rabbit IgG secondary antibody peroxidase conjugated | Rockland | Cat#611-1302; RRID: AB_219720 |
| Goat IRDye 800CW anti-mouse IgG secondary antibody | LI-COR | Cat#926-32210; RRID: AB_621842 |
| Human monoclonal anti-CD14, PE | BD Biosciences | Cat#555398; RRID: AB_395799 |
| Human monoclonal anti-CD11b, APC | Life Technologies | Cat#CD11B05; RRID: AB_1464195 |
| Human monoclonal anti-CD13, APC | Life Technologies | Cat#MHCD1305; RRID: AB_1464860 |
| Human monoclonal anti-CD33, PE | BD Pharmingen | Cat#555450; RRID: AB_395843 |
| Human monoclonal anti-CD45, PerCP-PB | Invitrogen | Cat#45-9459-42; RRID: AB_1548697 |
| Human/mouse monoclonal anti-CD11b, PB | BioLegend | Cat#101224; RRID: AB_755986 |
| Mouse monoclonal Ly-6G/Ly-6C, PeCy5 | Invitrogen | Cat#15-5931-82; RRID: AB_468813 |
| Mouse monoclonal anti-CD115, APC | BioLegend | Cat#135510; RRID: AB_2085221 |
| Mouse monoclonal anti-F4/80, PeCy7 | BioLegend | Cat#123114; RRID: AB_1626278 |
| Mouse monoclonal anti-CD45 | BD Pharmingen | Cat#559864; RRID: AB_398672 |
| Anti-Annexin V, APC | BD Biosciences | Cat#550475; RRID: AB_2868885 |
| Mouse monoclonal anti-CD3, PeCy5 | Invitrogen | Cat#15-0031-83; RRID: AB_468691 |
| Mouse monoclonal anti-CD4, PeCy5 | Invitrogen | Cat#15-0041-83; RRID: AB_468696 |
| Mouse monoclonal anti-CD8a, PeCy5 | Invitrogen | Cat#15-0081-83 RRID: AB_468707 |
| Mouse monoclonal anti-B220, PeCy5 | Invitrogen | Cat#15-0452-83; RRID: AB_468756 |
| Mouse monoclonal anti-TER-119, PeCy5 | Invitrogen | Cat#15-5921-83; RRID: AB_468811 |
| Mouse monoclonal anti-Ly-6A/E (Sca-1) | BioLegend | Cat#122520; RRID: AB_2143237 |
| Mouse monoclonal anti-Ly-6G/Ly-6C, FITC | BD Biosciences | Cat#553127; RRID: AB_394643 |
| Mouse monoclonal anti-Ly-6G/Ly-6C, APC-Cy7 | BioLegend | Cat#108424; RRID: AB_2137485 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Patient-derived xenographs (PDX) | Hematologic Oncology Tissue Bank at Memorial Sloan Kettering Cancer Center | N/A |
| Primary AML patient cells | Hematologic Oncology Tissue Bank at Memorial Sloan Kettering Cancer Center | N/A |
| Normal bone marrow cells | Hematologic Oncology Tissue Bank at Memorial Sloan Kettering Cancer Center | N/A |
| Cord blood | National Cord Blood Program, NY Blood Center | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| MG132 | Selleck Chemicals | Cat#S2619; CAS 1211877-36-9 |
| MLN4924 | Selleck Chemicals | Cat#S7109; CAS 905579-51-3 |
| CC-885 | Selleck Chemicals | Cat#S7109; CAS 1010100-07-8 |
| Lenalidomide | Sigma-Aldrich | Cat#SML2283; CAS 191732-72-6 |
| Pomalidomide | Sigma-Aldrich | Cat#P0018; CAS 19171-19-8 |
| complete, EDTA-free protease inhibitor cocktail | Sigma-Aldrich | Cat#11873580001 |
| MTT | Sigma-Aldrich | Cat#475989; CAS 298-93-1 |
| Cell lysis buffer | Cell Signaling Technology | Cat#9803S |
| Sequencing-grade trypsin | Promega | Cat#V5111 |
| Midostaurin | Selleck Chemicals | Cat#S8064; CAS 120685-11-2 |
| Cytarabine (U-19920A) | Selleck Chemicals | Cat#S1648; CAS 147-94-4 |
| Venetoclax | Selleck Chemicals | Cat#S8048; CAS 1257044-40-8 |
| Methothrexate | Selleck Chemicals | Cat#S1210; CAS 59-05-2 |
| Propium Iodide | Sigma-Aldrich | Cat#P4170; CAS 25535-16-4 |
| Murine SCF | Peprotech | Cat# 250-03 |
| Murine IL-3 | Peprotech | Cat# 213-13 |
| Murine IL-6 | Peprotech | Cat# 216-16 |
| Murine GM-CSF | Peprotech | Cat# 315-03 |
| MethoCult M3434 | Stem Cell Technologies | Cat# 03434 |
| MethoCult H4434 | Stem Cell Technologies | Cat# 04434 |
| Critical commercial assays | ||
| Luna Universal One-Step RT-qPCR Kit | New England BioLabs | Cat#E3005L |
| RNeasy Mini Kit | Qiagen | Cat#74104 |
| HTRF Human Cereblon Binding Kit | PerkinElmer | Cat#64BDCRBNPEG |
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | Cat#G7570 |
| QuikChange Lightning Multi Site-Directed Mutagenesis Kit | Agilent | Cat#210513 |
| QuikChange Lightning Site-Directed Mutagenesis Kit | Agilent | Cat#210518 |
| Dynabeads mRNA Purification Kit | Thermo Fisher Scientific | Cat#61006 |
| Monarch Total RNA Miniprep Kit | New England BioLabs | Cat#T2010S |
| TMTpro 16plex Label Reagent Set | Thermo Fisher Scientific | Cat#A44520 |
| Strep-Tactin Alpha Donor Beads | PerkinElmer | Cat#AS106D |
| AlphaLisa Nickel Chelate Acceptor Beads | PerkinElmer | Cat#AL108M |
| Richard-Allan Scientific Three Step Stain Set | Thermo Fisher Scientific | Cat#3300 |
| Deposited data | ||
| RNA-seq Data | GEO | GSE220707 |
| Proteomics Data | PRIDE Repository | PXD039474 |
| Experimental models: Cell lines | ||
| Human: MOLM-13 cells | Gary Gilliland Lab | RRID: CVCL_2119 |
| Human: MOLM-14 cells | Gary Gilliland Lab | RRID: CVCL_7916 |
| Human: OCI-AML3 cells | Gary Gilliland Lab | RRID: CVCL_1844 |
| Human: OCI-AML5 cells | Uli Steidl Lab | RRID: CVCL_1620 |
| Human: Kasumi-1 cells | Stephen Nimer Lab | RRID: CVCL_0589 |
| Human: NB-4 cells | Gary Gilliland Lab | RRID: CVCL_0005 |
| Human: KG-1A cells | Gary Gilliland Lab | RRID: CVCL_1824 |
| Human: NOMO-1 cells | Gary Gilliland Lab | RRID: CVCL_1609 |
| Human: HEK293T cells | ATCC | RRID: CVCL_0063 |
| Murine MLL-AF9 Crbn I391V leukemic cells | This paper | |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6-Crbntm1.1Ble/J | The Jackson Laboratory | Stock No: 032487 |
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) | The Jackson Laboratory | Stock No: 005557 |
| Mouse: C57BL/6J | The Jackson Laboratory | Stock No: 000664 |
| Oligonucleotides | ||
| Primers for RT-qPCR, See Table S7 | This paper | N/A |
| sgRNA 1: TP53 GATCCACTCACAGTTTCCAT | This paper | N/A |
| sgRNA 2: TP53 GAGCGCTGCTCAGATAGCGA | This paper | N/A |
| Recombinant DNA | ||
| pLC-Flag-CSNK1A1 | (Patil et al., 2018) | Addgene #123319 |
| pMSCV-IRES-RFP(MIR)-mouse IKZF2 | (Park et al., 2019) | |
| pHFUW-human IKZF2-IRES-GFP | This paper | N/A |
| Software and algorithms | ||
| Proteome Discoverer version 2.4.1.15 | Thermo Fisher Scientific | https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/multi-omics-data-analysis/proteome-discoverer-software.html |
| ImageJ | https://imagej.nih.gov/ij/ | |
| ChemDraw Professional 16.0 | PerkinElmer | https://perkinelmerinformatics.com/products/research/chemdraw |
| Mnova x64 | Mestrelab Research | https://mestrelab.com/download/mnova/ |
| Molecular Operating Environment | Chemical Computing Group | https://www.chemcomp.com/index.htm |
| Prism 7.0 | GraphPad | https://www.graphpad.com/ |
| Enrichr | Chen et al. | https://maayanlab.cloud/Enrichr/ |
| FlowJo 10.8.1 | Becton Dickinson & Company (BD) | https://www.flowjo.com/ |
| Other | ||