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. 2023 Aug 18;66:102857. doi: 10.1016/j.redox.2023.102857

Fig. 1.

Fig. 1

IR induces ferroptosis in mice jejunum. (A) Representative images of H&E staining of jejunum sections from control and irradiated mice at D4 after 11 Gy ABI. Scale bar, 100 μm. (B) Radio of the villus height to crypt depth in jejunum sections at D4 after 11 Gy ABI (n = 3). (C) Differentially expressed genes in jejunum samples harvested from control and RIII mice. (D) KEGG pathway enrichment analysis enriched by DEGs. (E) The heatmap of ferroptosis-related genes expression in jejunum samples from control and RIII mice (n = 3). (F) Western blot of GPX4 in the jejunum samples from control mice and irradiated mice at the indicated times post 11 Gy of ABI. Quantification of GPX4 expression is depicted in (G). (H) Representative immunohistochemistry images of 4-HNE in the jejunum sections of control and irradiated mice at D4 after 11 Gy of ABI. Scale bar, 50 μm. Quantification of the 4-HNE staining is depicted in (I), n = 3. (J) MDA levels in the jejunum samples from control and irradiated mice at D2 after 11 Gy of ABI (n = 3). *: p < 0.05, **: p < 0.01.