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. 2023 Aug 18;66:102857. doi: 10.1016/j.redox.2023.102857

Fig. 4.

Fig. 4

Radiation induces ferroptosis via upregulating ACSL4 expression. (A) Western blot of ACSL4 in the jejunum samples from control and irradiated mice at 48 h post 11 Gy ABI. Quantification of ACSL4 expression is depicted in (B). (C) Western blot of ACSL4 in FHs74Int cells at 48 h post 10 or 20 Gy irradiation with or without AA (40 μM) treatment. (D) Quantitative PCR detection of the relative expression of ACSL4 transcripts in FHs74Int cells at indicated time points post 10 Gy irradiation in the presence of AA (40 μM). (E) Western blot of ACSL4 in FHs74Int cells at 48 h following siRNA transfection. (F) Percentage of dead NC or siACSL4 FHs74Int cells, treated with DMSO or Fer-1 (5 μM), detected at 120 h after IR in the presence of AA (40 μM). (G) Lipid peroxidation levels in NC or siACSL4 FHs74Int cells, treated with DMSO or Fer-1 (5 μM), detected at 72 h after IR in the presence of AA (40 μM). (H) Percentage of dead FHs74Int cells, treated with indicated concentrations of rosiglitazone, detected at 120 h after 10 Gy irradiation in the presence of AA (40 μM). (I) Lipid peroxidation levels in FHs74Int cells, treated with indicated concentrations of rosiglitazone, detected at 72 h after 10 Gy irradiation in the presence of AA (40 μM). *: p < 0.05, **: p < 0.01, ***: p < 0.001.