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. 2023 Aug 18;66:102857. doi: 10.1016/j.redox.2023.102857

Fig. 5.

Fig. 5

Radiation upregulates ACSL4 expression via STAT1/IRF1 axis. (A) Protein expression level of pSTAT1/STAT1 in FHs74Int cells detected at 48 h post 10 or 20 Gy irradiation in the presence of AA (40 μM). (B) Protein expression level of ACSL4 and pSTAT1/STAT1 in NC or siSTAT1 FHs74Int cells detected at 48 h post 10 Gy irradiation in the presence of AA (40 μM). (C) Quantitative PCR detection of the relative expression of ACSL4 transcripts in NC or siSTAT1 FHs74Int cells at 48 h post irradiation. (D) Percentage of dead NC or siSTAT1 FHs74Int cells, treated with DMSO or Fer-1 (5 μM), detected at 120 h after irradiation in the presence of AA (40 μM). (E) Lipid peroxidation levels in NC or siSTAT1 FHs74Int cells, treated with DMSO or Fer-1 (5 μM), detected at 72 h after irradiation in the presence of AA (40 μM). (F) Protein expression level of IRF1 and pSTAT1/STAT1 in NC or siSTAT1 FHs74Int cells detected at 48 h post irradiation in the presence of AA (40 μM). (G) Protein expression level of ACSL4 and IRF1 in NC and siIRF1 FHs74Int cells detected at 48 h post irradiation in the presence of AA (40 μM). (H) Quantitative PCR detection of the relative expression of ACSL4 transcripts in NC and siIRF1 FHs74Int cells at 48 h post irradiation. (I) Percentage of dead NC and siIRF1 FHs74Int cells, treated with DMSO or Fer-1 (5 μM), detected at 120 h after irradiation in the presence of AA (40 μM). (J) Lipid peroxidation levels in NC and siIRF1 FHs74Int cells, treated with DMSO or Fer-1 (5 μM), detected at 72 h after irradiation in the presence of AA (40 μM). *: p < 0.05, **: p < 0.01, ***: p < 0.001.