FIGURE 4.

Procedure for proteomic data analysis and criteria for protein selection validation. In a first step, dia‐PASEF analysis of human AC samples revealed 2153 proteins. After applying restricted condition of FC > 1.5 and p value <0.05, 178 proteins were identified as DEPs and cell type expression, SynGo and Metascape analyses were performed (data shown in Tables 3, 4, 5). Then, literature review of DEPs was carried out in order to select proteins for validation. Proteins were chosen based on three main criteria: previous evidence linking protein and AD must be reported; proteins widely described in the disease were excluded; and potential relation or expression in the studied cell types (neurons, microglia, and astrocytes) was also considered.