Table 5.

Drugs used to validate PLATE-Seq and the IMR-32 cell line for connectivity analysis. Not all compounds of a given mechanism of action will necessarily map to that mechanism’s associated PCL—PCLs consist of compounds that are members of the same functional class and also have high transcriptional impact.

Drug	Mechanism of Action	CMap Perturbagen Class (PCL)
Mibefradil	T-type Ca${}^{2+}$ channel inhibitor	CP_T_TYPE_CALCIUM_CHANNEL_BLOCKER
Isradipine	L-type Ca${}^{2+}$ channel inhibitor	CP_CALCIUM_CHANNEL_BLOCKER
Nifedipine	L-type Ca${}^{2+}$ channel inhibitor	CP_CALCIUM_CHANNEL_BLOCKER
Diltiazem	Ca${}^{2+}$ channel inhibitor	CP_CALCIUM_CHANNEL_BLOCKER
Verapamil	Ca${}^{2+}$ channel inhibitor	CP_CALCIUM_CHANNEL_BLOCKER
Fendiline	Ca${}^{2+}$ channel inhibitor	CP_CALCIUM_CHANNEL_BLOCKER
Topiramate	Na${}^{+}$ and Ca${}^{2+}$ channel modulator	CP_SODIUM_CHANNEL_BLOCKER
Ionomycin	Ca${}^{2+}$ channel signal inducer
1-EBIO	Ca${}^{2+}$-gated K${}^{+}$ channel activator	CP_POTASSIUM_CHANNEL_ACTIVATOR
Forskolin	Adenylyl cyclase activator
Pregabalin	Increases GABA biosynthesis
Gabapentin	Increases GABA biosynthesis
Baclofen	GABA${}_B$-receptor agonist
Memantine	Glu-receptor inhibitor
Acamprostate	Glu-receptor inhibitor	CP_GABA_RECEPTOR_ANTAGONIST
MTEP	Glu-receptor inhibitor
Ivermectin	Glu-gated Cl${}^{-}$ channel inhibitor
Carbenoxolone	Glucocorticoid metabolism inhibitor
Mifepristone	Glucocorticoid receptor inhibitor	CP_PROGESTERONE_RECEPTOR_ANTAGONIST
Dexamethasone	Glucocorticoid receptor agonist	CP_GLUCOCORTICOID_RECEPTOR_AGONIST
Aldosterone	Mineralocorticoid receptor agonist
Spironolactone	Mineralocorticoid receptor inhibitor
Olanzapine	Dopamine receptor inhibitor	CP_DOPAMINE_RECEPTOR_ANTAGONIST
Eticlopride	Dopamine receptor inhibitor	CP_DOPAMINE_RECEPTOR_ANTAGONIST
Ondansetron	5-HT${}_3$ serotonin receptor inhibitor	CP_SEROTONIN_RECEPTOR_AGONIST
Naltrexone	Opioid receptor inhibitor
Disulfiram	Acetaldehyde dehydrogenase inhibitor
Cerlitinib	ALK inhibitor
Crizotinib	ALK inhibitor
Sirolimus	mTOR inhibitor	CP_MTOR_INHIBITOR
Manumycin A	Farnesyltransferase inhibitor	CP_NFKB_PATHWAY_INHIBITOR
Vorinostat	HDAC (I/II/IV) inhibitor	CP_HDAC_INHIBITOR
Prazosin	Adrenergic receptor inhibitor	CP_BETA_ADRENERGIC_RECEPTOR_AGONIST
Rolipram	Phosphodiesterase-4 inhibitor
Minocycline	NOS inhibitor
Pioglitazone	PPAR$\gamma$/$\alpha$ inhibitor	CP_PPAR_RECEPTOR_AGONIST
Fenofibrate	PPAR$\alpha$ agonist	CP_PPAR_RECEPTOR_AGONIST