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Spot test of serial diluted toxins on plates with or without inducing agents for the PfdeA—RSB12 strategy. E. coli EPI400 harboring the successfully cloned toxins P1Doc, P1DocH66Y, EcMazF, EcMazFE24A, EcParE2, VcHigB2, barnase and FCcdB on the pJYP1 vector was spotted on LB agar plates supplemented with ampicillin and additionally vitamin B12 (A, OFF-state) or naringenin (B, ON-state) or a combination of both (C, partly induced) or none of the additional agents (D, partly induced).
