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Spot test of serial diluted toxins on plates with or without inducing agents for the Ptac—RStheo strategy. E. coli EPI400 harboring the successfully cloned toxins P1Doc*, P1DocH66Y, EcMazF, EcMazFE24A, EcParE2, VcHigB2, barnase* and FCcdB* on the pJYP3 vector were spotted on LB agar plates supplemented with ampicillin (A, OFF-state) and additionally theophylline and IPTG (B, ON-state) or IPTG alone (C, partly induced) or theophylline alone (D, partly induced).
