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Spot test of serial diluted E. coli EPI400 harboring pJYP4_VcParE2 on plates with or without inducing agents for the PBAD—RStheo strategy. E. coli EPI400 harboring pJYP4_VcParE2 was spotted on (A, OFF-state) LB agar plates supplemented with ampicillin and (B, ON-state) theophylline and arabinose.
