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IMAC purification of P1DocH66Y (blue) and P1Doc* (red) using the Ptac—RStheo strategy. Fractions collected during IMAC elution (A,B) were analyzed through 20% SDS-PAGE (C,D) and anti-histidine Western blot (E,F). The blue and red arrows respectively indicate P1DocH66Y and P1Doc* (14.6 kDa).
