Table 3.

Plasmids used in this study. Toxins cloned in pJYP1 are P1Doc, P1Doc^H66Y, EcMazF, EcMazF^E24A, EcParE2, VcHigB2, barnase and FCcdB. For pJYP2: P1Doc*, P1Doc^H66Y, EcMazF, EcMazF^E24A, EcParE2, VcHigB2, barnase* and FCcdB*. For pJYP3: P1Doc^H66Y, EcMazF and EcMazF^E24A.

Plasmids	Description
pJYP1_toxin	FdeR—PfdeA—RSB12—toxin in HpaI/XhoI site of pET22b
pJYP2_toxin	LacI—Ptac—RStheo—toxin in BglII/XhoI site of pET22b
pJYP3_toxin	LacI—Ptac—RSB12—toxin in BglII/XhoI site of pET22b
pJYP4_VcParE2	AraC—PBAD—RStheo—VcParE2 in BglII/XhoI site of pET22b