Table 2.

Size and Mass Analysis of the RSV F/PS80 Nanoparticle

	DLS		SV-AUCa		SANSb/SAXS
	Z-ave (nm)	PDI	s20,Wc (Svedbergs)	Mwd (kDa)	Dmax (Å)	Rg (Å)	Mw (kDa)
RSV F/PS80	25–52	0.1–0.2	27.04	1200	500–550	145–170	1419 ± 20
RSV F component					500	145–153	965 ± 23
PS80 component					300	84–90	454 ± 6
Free PS80 control			2.0		100	32–47

^aSV-AUC results calculated from absorbance detection at 280 nm.

^bSANS values reported for samples in 99% D₂O buffer, thus accounting for both RSV F protein and PS80 detergent scattering contributions. RSV F and PS80 component contributions are from SANS data at the respective D₂O match point.

^cs20,W values are reported as weighted average sedimentation coefficient.

^dM_w value is calculated using a partial specific volume of 0.81 cm³ g⁻¹ and reported using D50 statistics.