Consequences of replacing FMN with deoxyFMN in T. neapolitana (TnIYD)^a.

TnIYD	X-Tyr	K D (μM)	k cat (×10−2 s−1)	K m (μM)	k cat/Km (×103 M−1 s−1)
FMN	I-Tyr	0.02 ± 0.1	2.7 ± 0.2b	0.22 ± 0.07b	120 ± 40b
	F-Tyrc	0.08 ± 0.03	NDd	—	—
deoxyFMN	I-Tyr	0.5 ± 0.1	6.2 ± 0.8	24 ± 5	2.6 ± 0.6
	F-Tyr	4.0 ± 0.4	ND	—	—

^aData represent an average of two independent measurements. Error for the kinetic parameter represents the range of values determined from each trial (see ESI).

^bValues from ref. 24.

^cValues from ref. 17.

^dND, not determined.