. 2023 Aug 1;10:1305. Originally published 2021 Dec 22. [Version 3] doi: 10.12688/f1000research.74506.3

Table 1. B cell subsets surface markers and corresponding compartments in MS.

Up-to-date reported B-cell subtypes with reference to the B cell lineage in MS . Sub-types from other inflammatory conditions are also mentioned. ⁸⁸ Abbreviations : SLO: secondary lymphoid organ; GC: germinal center; BM: bone marrow; ASC: antibody secreting cells; LLPC: long lived plasma cells. ‘Compartment’ makes reference to the organ where the biomarkers were found.

B-cell subtype	Surface biomarkers	Compartment	Reference
Stem cell	CD34, HLA-DR	BM	12
Pro-B cell	CD19 ⁺CD34 ⁺IgM ^-	BM	20
Pro-B cell	CD34, HLA-DR, CD19	BM	12
Pre-B cell	HLA-DR, CD19, CD20, Pre-BCR	BM	12
Pre-B cell	CD19 ⁺CD34 ⁺IgM ^-	BM	20
Immature B cell	HLA-DR, CD19, CD20, IgM	BM	12
Transitional B cells	CD19 ⁺CD27 ^-CD38 ^hiCD24 ^hi	Blood	27
	HLA-DR, CD19, CD20, IgM, IgD, CD38	Blood	12
	CD19 ⁺IgD ⁺CD27 ^-CD38 ⁺	Spleen/blood	20
	CD19 ⁺CD38 ⁺⁺CD24 ⁺⁺	Spleen/blood	20
	CD24 ^highCD38 ^high	Blood, SLO	85
	CD38 ⁺⁺CD10 ⁺IgD ⁺	Blood	88
Naïve B cell	HLA-DR, CD19, CD20, IgM, IgD	SLO/Blood	12
	CD19 ⁺CD27 ^-	CSF	21
	CD19 ⁺CD27 ^-IgD ⁺	Blood	27
	CD19 ⁺IgD ⁺CD27 ⁺	Spleen/blood	20
	CD19 ⁺CD27 ^-IgD ⁺	CSF	97
	CD27 ^-IgD ⁺	Blood	88
Non class-switched memory B cell	CD19 ⁺IgD ⁺CD27 ⁺	Spleen/blood	20
Pre-class switched memory B cell	CD27 ⁺IgD ⁺	Blood	88
Memory B cell	CD19 ⁺CD27 ⁺IgD ^-	Blood	27
	HLA-DR, CD19, CD20, CD27	Blood, SLO	12
	CD19 ⁺CD27 ⁺	CSF	21
	CD19 ⁺CD27 ⁺CD80 ⁺CD86 ⁺	CSF	97
Centroblast	CD19 ⁺CD38 ⁺CD77 ⁺Ki67 ⁺Bcl-2 ^-	CSF	97
Centrocyte	CD19 ⁺CD38 ⁺CD77 ^-	CSF	97
Post-class witched memory B cell	CD27 ⁺IgD ^-	Blood	88
Class-switched Memory B cell	CD19 ⁺IgD ^-CD27 ⁺	Spleen/blood	20
Short lived plasmablast	CD19 ⁺CD27 ⁺⁺CD138 ⁺CD38 ⁺HLA-DR ⁺⁺	CSF	21
Plasmablasts	HLA-DR, CD19, CD20 ^low, CD27, CD38	SLO/blood	12
	CD19 ⁺CD27 ⁺CD38 ⁺	Blood	27
	CD38 ⁺⁺CD27 ⁺⁺IgD ^-	Blood	88
	CD19 ⁺CD27 ⁺CD38 ⁺⁺	Spleen/blood	20
	CD19 ⁺CD138 ⁺⁺	Spleen/blood	20
	CD19 ⁺CD27 ⁺⁺CD138 ⁺CD38 ⁺⁺	CSF	21
	CD19 ^dimCD27 ^hiCD38 ⁺CD138HLA-DR Ki67	CSF, blood	40
Plasma cells	CD19 ⁺CD27 ⁺⁺CD138 ⁺CD38 ^-	CSF	21
	CD19 ^low, CD27, CD38, CD138	SLO/blood	12
	CD38 ⁺CD138 ⁺	Spleen/blood	20
	CD19 ^-CD138 ⁺	CSF	97
	CD19 ⁺CD138 ⁺	Blood	27
Bregs cell	IL10, CD27, CD5, CD25, CD86, CD24, CD38	Blood	27
	CD24 ^highCD27 ⁺	Blood, SLO	85
	CD19 ⁺CD24 ⁺⁺CD38 ⁺⁺	Spleen/blood	20
	CD19 ⁺CD5 ⁺CD1d ⁺⁺	Spleen/blood	20
Double Negative B cell	CD27 ^-IgD ^-	Blood	88
LLPC	CD19 ^-IgD ^-CD27 ⁺CD138 ⁺CD38 ^Hi	BM	103
LLPC	CD19 ^-CD138 ⁺CD38 ^Hi	BM	103
ASC in CSF	CD19 ^-CD27 ⁺CD38 ⁺IgG	CSF	40
B cells in CSF	CD19 ⁺CD138 ^-	CSF	21
B cells in CSF	CD19 ^dimCD27 ^hiCD138 ⁺ASC	CSF	40