Table 2. Assay performance quality metrics.

Metric	Details
RNA Quantity	50–200 ng input
RNA Quality	≥30% DV200
DNA Quantity	150–750 ng input
DNA Quality	A260/230 1.7–2.9
Tumor Content	≥20% input
Library Quantification (bp)	195–350 bp
Onboard Q30	≥75%
PhiX	~0.2–1%
Depth of coverage	Median 500×, Boosted region >1500×, Normal DNA 150×
Total reads (RNA)	200 million